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ubch5a enzyme  (Boston Biochem)

 
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    Boston Biochem ubch5a enzyme
    Ubch5a Enzyme, supplied by Boston Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ubch5a enzyme/product/Boston Biochem
    Average 90 stars, based on 1 article reviews
    ubch5a enzyme - by Bioz Stars, 2026-03
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    (A) Schematic representation of ubiquitylated residues on TZF1 predicted by an online tool http://systbio.cau.edu.cn/araubisite . (B) TZF1 is ubiquitinated in vivo. Arabidopsis protoplasts were co-expressed with indicated constructs and IP was performed using anti-HA antibody and Western blot was carried out using anti-GFP antibody. (C) Same IP experiment with the addition of ubiquitin-activating enzyme E1 inhibitor PYR41 was carried out. (D) KEG ubiquitinates TZF1 in vitro . The in vitro reaction was carried out using recombinant E1, E2, and E3 (MBP-KEG) enzymes, ubiquitin, and GST-TZF1.

    Journal: bioRxiv

    Article Title: Modulation of stress granule dynamics by phosphorylation and ubiquitination in plants

    doi: 10.1101/2024.02.20.581253

    Figure Lengend Snippet: (A) Schematic representation of ubiquitylated residues on TZF1 predicted by an online tool http://systbio.cau.edu.cn/araubisite . (B) TZF1 is ubiquitinated in vivo. Arabidopsis protoplasts were co-expressed with indicated constructs and IP was performed using anti-HA antibody and Western blot was carried out using anti-GFP antibody. (C) Same IP experiment with the addition of ubiquitin-activating enzyme E1 inhibitor PYR41 was carried out. (D) KEG ubiquitinates TZF1 in vitro . The in vitro reaction was carried out using recombinant E1, E2, and E3 (MBP-KEG) enzymes, ubiquitin, and GST-TZF1.

    Article Snippet: The in vitro ubiquitination reaction was performed in a 30 μl mixture containing 200 ng E1 enzyme (BB-E-304-050, Boston Biochem), 200 ng E2 enzyme (BB-E2-616-100, Boston Biochem), 5 mg His-ubiquitin (BB-U-530, Boston Biochem), 2 mg purified MBP-KEG fusion protein (as E3 enzyme), and GST-TZF1 fusion protein in a reaction buffer that contains 50 mM Tris–HCl [pH 7.6], 2 mM DTT, 5 mM MgCl 2 , and 2 mM ATP.

    Techniques: In Vivo, Construct, Western Blot, Ubiquitin Proteomics, In Vitro, Recombinant